Efek Insektisida Aromaterapi Minyak Atsiri Daun Nilam (Pogostemon cablin Beth) Terhadap Aedes aegypti
The Insecticide Effect of Patchouli leaves (Pogostemon cablin Beth) Essential Oil Aromatherapy Candle Against Aedes aegypti
DOI:
https://doi.org/10.58623/aspirator.v16i1.84Keywords:
aromatherapy candles, insecticide, Pogostemon cablin, Aedes aegyptiAbstract
Aromatherapy candles are a safe and effective insecticide innovation with a long-lasting fragrance. Aromatherapy candles can also act as an adulticide for mosquitoes if made from the essential oil of patchouli leaves that have natural insecticidal properties against Aedes aegypti mosquitoes. This research aims to determine the adulticidal effect of patchouli leaves (Pogostemon cablin Benth) essential oil aromatherapy candle against Ae. aegypti mosquitoes. This research is experimental with a post-test only control group design divided into six groups: one negative control group and five treatment groups (25, 40, 55, 70, and 85%) with four repetitions. Each group consisted of 20 Ae. aegypti mosquitoes. Before testing, the aromatherapy candle was lit in the test room for three hours and then extinguished. The mosquitoes were exposed to the vapor produced by patchouli essential oil aromatherapy candles for five hours, and the mortality rate was assessed every hour. Afterward, the data were analyzed using One-way ANOVA, post hoc Bonferroni and probit analyses. The average mosquito mortality rate was 0% in the negative control group and 25; 32.5; 51.25; 70; and 87.5% in the treatment groups of 25, 40, 55, 70, and 85%, respectively. The data analysis indicated a significant difference in the mosquito mortality rate in all treatment groups (p<0.05) with a 95% confidence interval. Meanwhile, the probit analysis showed an LC50 of 63% and LC90 of 96.067%. We conclude that the aromatherapy candle made from patchouli essential oil had an adulticidal effect on Ae. aegypti mosquitoes.
References
WHO. 2024b. Dengue-Global Situation. Geneva: WHO. https://www.who.int/emergencies/disease-outbreak-news/item/2024-DON518 (2024).
Haider N, Hasan MN, Onyango J, dan Asaduzzaman M. Global Landmark: 2023 Marks the Worst Year for Dengue Cases with Millions Infected and Thousands of Deaths Reported.” IJID Regions. 2024;13:100459. doi: 10.1016/j.ijregi.2024.100459. DOI: https://doi.org/10.1016/j.ijregi.2024.100459
WHO. 2024a. Dengue and Severe Dengue. Geneva: WHO https://www.who.int/news-room/fact-sheets/detail/dengue-and-severe-dengue (2024).
WHO. 2022. Report on Insecticide Resistance in Aedes Mosquitoes (Aedes Aegypti, Ae. Albopictus, Ae. Vittatus) in WHO South-East Asia Region Countries. South-East Asia Region countries: WHO South East Asia.
Hassan MR, Azit NA, Fadzil SM, Ghani SRA, Ahmad N, dan Nawi AM. Insecticide Resistance of Dengue Vectors in South East Asia: A Systematic Review. Af Health Sci. 2021;21(3):1124–40. doi: 10.4314/ahs.v21i3.21 DOI: https://doi.org/10.4314/ahs.v21i3.21
Kamaraj C, Kumar RCS, Al-Ghanim KA, Nicoletti M, Sathiyamoorthy V, Sarvesh S, et al. Novel Essential Oils Blend as a Repellent and Toxic Agent against Disease-Transmitting Mosquitoes. Toxics. 2023.11(6):517. https://doi.org/10.3390/toxics11060517 DOI: https://doi.org/10.3390/toxics11060517
Jafar SH, Daud NS, Badia E, Wulaisfan R, dan Tee SA. Effectiveness of Combination of Crew (Cymbopogon citrtus) and Patchouli (Pongostemon cablin Benth) essential Oils with Cooter Oil as A Base. Warta Farmasi. 2023;12(2):37–44.
Mustapa MA, Guswenrivo I, Zurohtun A, Ikram NKI, dan Muchtaridi M. Analysis of Essential Oils Components from Aromatic Plants Using Headspace Repellent Method against Aedes Aegypti Mosquitoes. Molecules. 2023.28(11):4269. doi: 10.3390/molecules28114269 DOI: https://doi.org/10.3390/molecules28114269
Fatima S, Farzeen I, Ashraf A, Aslam B, Ijaz MU, Hayat S, et al. A Comprehensive Review on Pharmacological Activities of Pachypodol: A Bioactive Compound of an Aromatic Medicinal Plant Pogostemon Cablin Benth. Molecules. 2023;28(8):3469. https://doi.org/10.3390/molecules28083469 DOI: https://doi.org/10.3390/molecules28083469
Popescu IE, Gostin IN, dan Blidar CF. An Overview of the Mechanisms of Action and Administration Technologies of the Essential Oils Used as Green Insecticides. AgriEngineering. 2024; 6(2):1195–1217. https://doi.org/10.3390/agriengineering6020068 DOI: https://doi.org/10.3390/agriengineering6020068
Yunus RN. Malik DYSR, Rahayu MS, Aulyah dan Afrini IM. Effectiveness of Repellent from Patchouli (Pogestemon Cablin) Varieties of Southeast Sulawesi against Aedes Aegypti. J Vector Borne Dis. 2024;61:167–75. doi: 10.4103/jvbd.jvbd_38_22 DOI: https://doi.org/10.4103/jvbd.jvbd_38_22
Manjesh K, Kundu A, Dutta A, Saha S, dan Neelakanthaiah BS. Bio-Insecticidal Nanoemulsions of Essential Oil and Lipid-Soluble Fractions of Pogostemon Cablin. Front Plant Sci. 2022;13:874221. doi: 10.3389/fpls.2022.874221 DOI: https://doi.org/10.3389/fpls.2022.874221
Galovičová L, Borotová P, Valková V, Ďúranová H, Štefániková J, Vukovic N, et al. Biological Activity of Pogostemon Cablin Essential Oil and Its Potential Use for Food Preservation. Agronomy. 2022;12(2):387. https://doi.org/10.3390/agronomy12020387 DOI: https://doi.org/10.3390/agronomy12020387
Santos LL, Brandão LB, Da Costa ALP, Martins RL, Rodrigues ABL, Lobato AA, et al. 2022. Bioinsecticidal and Pharmacological Activities of the Essential Oil of Pogostemon Cablin Benth Leaves: A Review. Pharmacogn Rev 16(32):139–45. doi:10.5530/phrev.2022.16.18 DOI: https://doi.org/10.5530/phrev.2022.16.18
Meryanti NLA, dan Rismawati N. Comperation of Repelent Power of Orange Leaf Extract (Citrus Aurantifolia) and Nilam Leaf Extract (Pogostemon Cablin Benth) Toward Aedes Aegypti Mosquito. J Public Health Phar. 2022;2(2):41–44. doi: https://doi.org/10.56338/jphp.v2i2.3879 DOI: https://doi.org/10.56338/jphp.v2i2.3879
Mubarak M, Yunus R, Supiati S, dan Suwarni S. Larvicidal and Repellent Potential of Patchouli Extract (Pogostemon Cablin) Varieties of Southeast Sulawesi for Aedes Aegypty Vector. Egy J Chem. 2022. 66(1):89–98. doi:10.21608/ejchem.2022.123730.5527 DOI: https://doi.org/10.21608/ejchem.2022.123730.5527
Costa TL, Santos RCD, Santos AA, Pimentel EDS, Lima E, Batista CV, et al. Lethal and Sublethal Effects of an Essential Oil-Based Emulsion of Patchouli, Pogostemon Cablin (Lamiaceae), on the Tomato Leafminer. Agriculture. 2023;13(8):1540. https://doi.org/10.3390/agriculture13081540 DOI: https://doi.org/10.3390/agriculture13081540
Utami FD, Setianto AB, dan Yuliani S. Aktivitas Repellent Formulasi Sediaan Spray Kombinasi Minyak Atsiri Serai (Cymbopogon winterianus), Daun Kemangi (Ocimum basilicum) dan Nilam (Pogostemon Cablin) Beserta Uji Preferensinya. J Ilm Ibnu Sina (JIIS): Ilmu Farmasi dan Kesehatan. 2021;6(1):87–97. https://doi.org/10.36387/jiis.v6i1.631 DOI: https://doi.org/10.36387/jiis.v6i1.631
Khatimah H, Walanda DK, Poba D, Ratman, dan Afadil. Activity of Mosquito Coils from the Solid Waste of Patchouli (Pogostemon Cablin Benth) with Addition of Torch Ginger (Etlingera Elatior) Flower. IOP Conf Ser Earth Environ Sci. 2024. 1355(1):012035. doi:10.1088/1755-1315/1355/1/012035 DOI: https://doi.org/10.1088/1755-1315/1355/1/012035
Harahap HY, Syarifuddin S, Pranata C, Diningsih A, dan Mora L. Kombinasi Minyak Atsiri Sereh (Cymbopogon citratus), Kulit Kayu Manis (Cinnamomum burmannii) dan Minyak Nilam (Pogostemon cablin) sebagai Pengikat Lilin Aromaterapi. Forte J. 2024;4(2):360–67. https://doi.org/10.51771/fj.v4i2.915 DOI: https://doi.org/10.51771/fj.v4i2.915
WHO. 2009. Guidelines for Efficacy Testing of Household Insecticide Products; Mosquitoe Coils, Vaporizer Mats, Liquid Vaporizer, Ambient Emanators, and Aerosols. 1st ed. Geneva.
Dewi AP, and Lusiyana N. Uji Daya Tolak Lilin Aromaterapi Minyak Atsiri Serai (Cymbopogon citratus) terhadap Nyamuk Aedes aegypti. Balaba: Jurnal Litbang Pengendalian Penyakit Bersumber Binatang Banjarnegara. 2020;16(1):21–28. doi: doi: 10.22435/blb.v16i1.2053 DOI: https://doi.org/10.22435/blb.v16i1.2053
Tutuarima T dan Antara YI. Kinerja Alat Penyulingan Minyak Atsiri Limbah Industri Sirup Kalamansi Skala Kecil Dengan Metode Steam Distillation. AGRITEKNO: Jurnal Tekno Pert,2020;9(2):42–47. https://doi.org/10.30598/jagritekno.2020.9.2.42 DOI: https://doi.org/10.30598/jagritekno.2020.9.2.42
Fransisca E, Wening DK, dan AI Shafira. Pembuatan Lilin Aromaterapi dengan Penambahan Minyak Serai (Cymbopogon Citratus) di Kelurahan Kalirejo, Kecamatan Ungaran Timur, Kabupaten Semarang.Ind J Com Enpower. 2022:4. doi: https://doi.org/10.35473/ijce.v4i2.1935
Sari PI, Farid N, Wahyuningsih S, dan Sari I. Formulasi dan Uji Efektivitas Spray Antinyamuk Kombinasi Minyak Sereh (Cymbopogon nardus) dan Minyak Nilam (Pogostemon cablin). J Buana Farma. 2022;2(4):1–9. doi: https://doi.org/10.36805/jbf.v2i4.605 DOI: https://doi.org/10.36805/jbf.v2i4.605
Buang A, Adriana AN, dan Rudi. Formulasi Lilin Aromaterapi Kombinasi Minyak Atsiri Daun Nilam (Pogostemon cablin Benth) dan Minyak Atsiri Daun Jeruk Nipis (Citrus aaurantifolia Swingle) sebagai Antinyamuk Aedes aegyti. Fito Med. 2022;14(1):9–18. doi: doi: https://doi.org/10.47650/fito.v14i1.496 DOI: https://doi.org/10.29103/cejs.v1i1.4891
Ariati W. Formulation and Effectiveness Testing of Mosquito Repellent Spray Combination of Citrusev Oil (Cymbopogon Nardus) and Patchouli Oil (Pogostemon Cablin). NSMRJ: Nusant Sci Med Res J. 2023;2(01):55–59. doi: https://doi.org/10.58549/nsmrj.v2i01.65 DOI: https://doi.org/10.58549/nsmrj.v2i01.65
Junren C, Xiaofang X, Mengting L, Qiuyun X, Gangmin L, Huiqiong Z, et al. Pharmacological Activities and Mechanisms of Action of Pogostemon Cablin Benth: A Review. Chin Med. 2021;16(1):5. doi: 10.1186/s13020-020-00413-y DOI: https://doi.org/10.1186/s13020-020-00413-y
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